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Preparation of a novel AAV-ITR gene expression mini vector in Sf9 insect cells via baculovirus / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1230-1238, 2015.
Article in Chinese | WPRIM | ID: wpr-240561
ABSTRACT
AAV-ITR gene expression mini vector is a double-strand or single-strand DNA that only contains inverted terminal repeats of adeno-associated virus, cis-elements and gene of interest and does not contain any other foreign DNA sequences. We prepared Bac-ITR-EGFP and Bac-inrep. Spodoptera frugiperda cells were infected with Bac-ITR-EGFP (P3) and Bac-inrep (P3). Up to 100 μg of AAV-ITR-EGFP gene expression mini vectors were extracted from 2 x 10(7) cells of Sf9 72 h after infection. The gel electrophoresis analysis shows that most forms of AAV-ITR-EGFP gene expression mini vector were monomer and dimer. The mini vector expression efficacy was examined in vitro with HEK 293T cells. The EGFP expression was observed at 24 h after transfection, and the positive ratio reached 65% at 48 h after transfection.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA, Single-Stranded / Transfection / Gene Expression / Baculoviridae / Dependovirus / Terminal Repeat Sequences / HEK293 Cells / Sf9 Cells / Genetic Vectors Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA, Single-Stranded / Transfection / Gene Expression / Baculoviridae / Dependovirus / Terminal Repeat Sequences / HEK293 Cells / Sf9 Cells / Genetic Vectors Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2015 Type: Article