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Expression of neuropeptide Y and long leptin receptor in gastrointestinal tract of giant panda / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1175-1183, 2015.
Article in Chinese | WPRIM | ID: wpr-240566
ABSTRACT
To study the expression and distribution of neuropeptide Y (NPY) and long leptin receptor (OB-Rb) in the gastrointestinal tract of giant panda, samples of three animals were collected from the key laboratory for reproduction and conservation genetics of endangered wildlife of Sichuan province, China conservation and research center for the giant panda. Paraffin sections of giant panda gastrointestinal tissue samples were observed using hematoxylin-eosin staining (HE) and strept actividin-biotin complex immunohistochemical staining (IHC). The results show that the intestinal histology of three pandas was normal and no pathological changes, and there were rich single-cell and multi-cell mucous glands, long intestinal villi and thick muscularis mucosa and muscle layer. Positive cells expressing NPY and OB-Rb were widely detected in the gastrointestinal tract by IHC methods. NPY positive nerve fibers and neuronal cell were widely distributed in submucosal plexus and myenteric plexus, especially in the former. They were arranged beaded or point-like shape. NPY positive cells were observed in the shape of ellipse and polygon and mainly located in the mucous layer and intestinal glands. OB-Rb positive cells were mainly distributed in the mucous layer and the laminae propria, especially the latter. These results confirmed that NPY and OB-Rb are widely distributed in the gut of the giant panda, which provide strong reference for the research between growth and development, digestion and absorption, and immune function.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Ursidae / Neuropeptide Y / China / Receptors, Leptin / Genetics / Intestines / Metabolism Limits: Animals Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Ursidae / Neuropeptide Y / China / Receptors, Leptin / Genetics / Intestines / Metabolism Limits: Animals Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2015 Type: Article