Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1050-1062, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-240597
ABSTRACT
Rhamnolipid biosurfactant is mainly produced by Pseudomonas aeruginosa that is the opportunistic pathogenic strain and not suitable for future industrial development. In order to develop a relatively safe microbial strain for the production of rhamnolipid biosurfactant, we constructed engineered Escherichia coli strains for rhamnolipid production by expressing different copy numbers of rhamnosyltransferase (rhlAB) gene with the constitutive synthetic promoters of different strengths in E. coli ATCC 8739. We further studied the combinatorial regulation of rhlAB gene and rhaBDAC gene cluster for dTDP-1-rhamnose biosynthesis with different synthetic promoters, and obtained the best engineered strain-E. coli TIB-RAB226. Through the optimization of culture temperature, the titer of rhamnolipd reached 124.3 mg/L, 1.17 fold higher than that under the original condition. Fed-batch fermentation further improved the production of rhamnolipid and the titer reached the highest 209.2 mg/L within 12 h. High performance liquid chromatography-mass spectrometry (LC-MS) analysis showed that there are total 5 mono-rhamnolipid congeners with different nuclear mass ratio and relative abundance. This study laid foundation for heterologous biosynthesis of rhanomilipd.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pseudomonas aeruginosa
/
Rhamnose
/
Surface-Active Agents
/
Bacterial Proteins
/
Industrial Microbiology
/
Glycolipids
/
Multigene Family
/
Promoter Regions, Genetic
/
Decanoates
/
Escherichia coli
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2015
Type:
Article
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