Enhancing erythromycin precursor 6-dEB production by using synthetic small regulatory RNAs in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1039-1049, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-240598
ABSTRACT
Although heterologous biosynthesis of polyketide erythromycin has been successfully achieved in Escherichia coli, the titer remains at a very low level (-10 mg/L). In this study, based on genome-scale metabolic model of E. coli, in silico method flux distribution comparison analysis was used to discover novel potential targets for heterologous 6-dEB biosynthesis. Synthetic small regulatory RNAs (sRNAs) was used to experimentally test 12 down-regulated targets. The results showed that repression of each of these target genes e.g. lsrC and ackA led to significantly improve heterologous 6-dEB biosynthesis. Using co-repression of lsrC and ackA, 6-dEB titer was improved by 59.9% in shake-flask with a maximum yield of 22.8 mg/L. This study indicates that combined flux distribution comparison analysis and synthetic small regulatory RNAs is an effective strategy to improve 6-dEB production in E. coli.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Industrial Microbiology
/
RNA
/
Down-Regulation
/
Erythromycin
/
Escherichia coli
/
Genetics
/
Metabolism
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2015
Type:
Article
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