Improvement of natamycin production in an industrial strain by heterologous expression of the afsRS(cla) global regulatory genes / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 744-751, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-240603
ABSTRACT
The afsRS(cla) global regulatory genes from Streptomyces clavuligerus activate the production of two antibiotics in Streptomyces lividans. In this study, we gained an increase of 38% in the production of natamycin (3.56 g/L) in an industrial strain Streptomyces gilvosporeus TZ1401 through the integration of pHL851 that bears the afsRS(cla) global regulatory genes into its genome. We discovered by quantitive real-time reverse transcription PCR (qRT-PCR) that the expression of 6 genes of the natamycin biosynthetic gene cluster were improved from 1.9 to 2.7 times. This suggests that afsRS(cla) improve the production of natamycin through increased transcription. This study provides a good example for applying afsRS(cla) in high yield breeding of industrial antibiotic producers.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Streptomyces
/
Industrial Microbiology
/
Genes, Regulator
/
Multigene Family
/
Natamycin
/
Genetics
/
Anti-Bacterial Agents
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2015
Type:
Article
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