Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 3616-3622, 2014.
Article
in English
| WPRIM
| ID: wpr-240718
ABSTRACT
<p><b>BACKGROUND</b>Dendritic cells (DCs) can recognize the pathogen-associated molecular patterns (PAMP) of Aspergillus fumigatus (A. fumigatus), activating the immune response. During A. fumigatus infection, a Th and Treg response induced in the fungi-pulsed DCs is not yet well understood.</p><p><b>METHODS</b>In this study, bone marrow-derived dendritic cells (BMDCs) were separated and proliferated from C57BL/6 mice. A. fumigatus pulsed DCs were generated and cultured with CD4(+) T cells derived from the spleen of C57BL/6 mice in vitro. CD4(+) T cells differentiation after co-culture were analyzed by flow cytometry, ELISA, and real-time PCR analysis.</p><p><b>RESULTS</b>The A. fumigatus pulsed DCs exhibited increased Th1 and Treg frequency, Th1-related cytokines (IFN-γ and IL-12), Treg-related cytokines (TGF-β) and T-bet, and Foxp3 mRNA levels compared with the control group. There was no significant difference between A. fumigatus pulsed DCs group and the control group about Th17 and Th2 frequency.</p><p><b>CONCLUSIONS</b>The inactivated conidia of A. fumigatus were able to activate BMDCs and made them capable of triggering T cell responses in vitro. A. fumigatus loaded DCs was a weak inducer of Th17 and Th2, but induced a strong Th1 and Treg response.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aspergillus fumigatus
/
Virulence
/
Dendritic Cells
/
Cytokines
/
Transforming Growth Factor beta
/
T-Lymphocytes, Regulatory
/
T-Lymphocytes, Helper-Inducer
/
Th1 Cells
/
Interleukin-12
/
Allergy and Immunology
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2014
Type:
Article
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