Experimental study on effect of gusongbao contained serum of old rats on osteoblast proliferation of rabbits / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of Gusongbao (GSB) on proliferation and metabolism of osteoblast cultured in vitro.</p><p><b>METHODS</b>Old rats, aged 18 months, were given GSB 1.5 g/kg, twice a day for 3 days by intragastric perfusion. Blood of the rats was collected 1 hr after the final perfusion to isolate serum for preparing, with D8900 medium, the culture media containing 7.5% or 15% GSB, which was used to culture osteoblast for 24 hrs. Besides, D8900 media containing 7.5% or 15% old rats'serum without medication, containing 20 mumol/L, sodium fluoride, and simple D8900 medium were prepared for control. The cell proliferation was detected by MTT method, and the changes of Ca2+ concentration and ALP content in supernatant of culture were also observed.</p><p><b>RESULTS</b>The osteoblast proliferation cultured in GSB serum containing medium was significantly increased than those cultured in the other control media (P < 0.01), at the same time, the Ca2+ consumption increased and the ALP content elevated significantly (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>GSB could promote the DNA synthesis, increase the utilization of Ca2+ and accelerate the growth and proliferation of osteoblast.</p>