Impact of adenovirus-mediated PDE5-shRNAs on the free calcium level in the smooth muscle cells of rat corpus cavernosum / 中华男科学杂志
National Journal of Andrology
;
(12): 882-885, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-241238
ABSTRACT
<p><b>OBJECTIVE</b>To observe the influence of recombinant adenovirus-mediated PDE5-shRNAs on the free calcium level in rat penile smooth muscle cells and to explore the feasibility of gene therapy for erectile dysfunction (ED).</p><p><b>METHODS</b>Smooth muscle cells of the rat corpus cavernosum were transfected with constructed rAd-rPDE5-shRNAs and then dyed with the calcium fluorescent probe Fluo-3/AM at 24, 48 and 72 hours. The dynamic changes of the calcium fluorescence intensity were observed under the laser scanning confocal microscope (LSCM). The relative level of intracellular calcium was determined by fluorescence indexes.</p><p><b>RESULTS</b>The fluorescence indexes of calcium at 24, 48 and 72 hours were 829.3 +/- 7.8, 801.5 +/- 9.5 and 856.3 +/- 8.7 in the rAd-rPDES-shRNAs group, significantly lower than in the rAd-mock (1106.3 +/- 10.8, 1121.3 +/- 10.2 and 1058.5 +/- 12.1) and blank control group (1076.6 +/- 9.7, 1133.4 +/- 11.2 and 1104.3 +/- 10.5) (P < 0.05).</p><p><b>CONCLUSION</b>Adenovirus mediated shRNAs of the target PDE5 gene can significantly decrease the intracellular calcium level in the smooth muscle cells of the rat corpus cavernosum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penis
/
Therapeutics
/
Genetic Therapy
/
Adenoviridae
/
Calcium
/
Rats, Sprague-Dawley
/
Gene Silencing
/
Cell Biology
/
Myocytes, Smooth Muscle
/
RNA, Small Interfering
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2009
Type:
Article
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