Recombinant human testis sperm binding protein increases sperm motility parameters / 中华男科学杂志
National Journal of Andrology
;
(12): 783-787, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-241257
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of recombinant human testis sperm binding protein (TSBP) on human sperm motility parameters in vitro.</p><p><b>METHODS</b>Sperm specimens obtained from 22 healthy fertile men were prepared by the Percoll gradient-centrifugation technique. The sperm suspension was incubated with recombinant His6-TSBP at the concentration of 0.01 mg/ml or 0.1 mg/ml at 37 degrees C for 1 or 3 hours in vitro. The combination of the recombinant protein and sperm membrane was determined by Western blot, and the sperm motility parameters were analyzed by computer-aided sperm analysis (CASA). The same procedure was performed for 12 asthenospermia patients.</p><p><b>RESULTS</b>In the 22 healthy volunteers, the percentage of forward motile sperm was increased after incubated with 0.1 mg/ml recombinant protein for 1 h (P < 0.05), both forward motile sperm percentage and motility were increased after incubated with recombinant protein at the same concentration for 3 h (P < 0.05), but no effect was observed after incubation with 0.01 mg/ml recombinant protein. In the 12 asthenospermia patients, the forward motile sperm percentage was increased after incubated with 0.1 mg/ml recombinant protein for 3 h (P < 0.05), but no statistically significant difference was observed in sperm motility.</p><p><b>CONCLUSION</b>Recombinant His6-TSBP at the concentration of 0.1 mg/ml can increase sperm motility in healthy fertile men and the forward motile sperm percentage in both healthy fertile men and asthenospermia patients in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sperm Motility
/
Recombinant Proteins
/
Seminal Plasma Proteins
/
Infertility, Male
/
Metabolism
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2009
Type:
Article
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