Nandeshi: a powerful inhibitor of human acrosin activity / 中华男科学杂志
National Journal of Andrology
;
(12): 700-702, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-241274
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the inhibitory effect of Nandeshi, an acrosin inhibitor, on human acrosin activity.</p><p><b>METHODS</b>We collected sperm samples from 10 healthy fertile men and cultured them with Nandeshi at 30 degrees C for 5 minutes at the concentrations of 0. 100, 0.120, 0.144, 0.173, 0.207, 0.249, 0.299, 0.358 and 0.430 mmol/L, with the controls treated with a well-known acrosin inhibitor N-alpha-p-tosyl-L-lysine chloromethylketone (TLCK) at 150.0, 189.8, 213.6, 240.3, 270.3, 304.1 and 342.1 mmol/L. Then we determined the residual activity of human acrosin by improved Kennedy assay.</p><p><b>RESULTS</b>The residual activity of acrosin was negatively correlated with the Nandeshi concentration, and Nandeshi exhibited an inhibition rate about 800 times that of TLCK.</p><p><b>CONCLUSION</b>Nandeshi has a powerful inhibitory effect on human acrosin, and improved Kennedy assay is a simple, practical and highly sensitive technique for the detection of human acrosin activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Spermatozoa
/
Tosyllysine Chloromethyl Ketone
/
Acrosin
/
Contraceptive Agents, Female
/
Enzyme Inhibitors
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2009
Type:
Article
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