Silencing nucleostemin expression reduces the proliferation of PC-3 cells / 中华男科学杂志
National Journal of Andrology
;
(12): 593-598, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-241295
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of the nucleostemin (NS) gene in prostate cancer PC-3, LNCaP and DU145 cells, and to study the effect of the NS gene on the proliferation of PC-3 cells after its silencing.</p><p><b>METHODS</b>The protein and mRNA expressions of NS in PC-3, LNCaP and DU145 cells were respectively detected by immunohistochemical staining and RT-PCR. An NS-specific short-hairpin RNA (shRNA) expression plasmid was used to transfect the PC-3 cells (NS-shRNA-PC-3), followed by observation of the changes of the NS gene and the proliferation and apoptosis of the cells.</p><p><b>RESULTS</b>The NS gene was highly expressed in the three types of cells. After the transfection, the NS expression and the proliferation of the NS-siRNA-PC-3 cells were remarkably reduced, while the percentage of the GO/G1 cells and the early apoptosis of the PC-3 cells obviously increased. A marked decrease was observed in the neoplasm forming ability of the NS-siRNA-PC-3 cells in the nude mice.</p><p><b>CONCLUSION</b>NS is highly expressed in prostate cancer cells. The proliferation of PC-3 cells is remarkably reduced and the early apoptosis of PC-3 cells increased after silencing the NS gene by NS-specific shRNA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostatic Neoplasms
/
Nuclear Proteins
/
Transfection
/
Carrier Proteins
/
Apoptosis
/
GTP-Binding Proteins
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Silencing
/
RNA, Small Interfering
/
Cell Line, Tumor
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2009
Type:
Article
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