Combination of short-period sperm-oocyte coincubation and early rescue intracytoplasmic sperm injection after total failure of in vitro fertilization / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the outcome of the combination of short-period sperm-oocyte coincubation with early rescue intracytoplasmic sperm injection (ICSI) after complete failure of in vitro fertilization (IVF).</p><p><b>METHODS</b>We used short-period sperm-oocyte coincubation and overnight fertilization for the oocytes obtained from 10 IVF-ET cycles, and compared the fertilization results and embryo quality. We conducted polar body observation following short-period sperm-oocyte coincubation for 105 conventional IVF cycles, and evaluated the clinical outcome of early rescue ICSI for total fertilization failure.</p><p><b>RESULTS</b>No significant differences were noted in fertilization outcome and embryo quality between the 3 h short-period and overnight fertilizations (P > 0.05). The combined strategy achieved a clinical pregnancy rate of 53.3%, an implantation rate of 38.0%, with 64 babies born, of whom 44.8% were taken home.</p><p><b>CONCLUSION</b>The combination of short-period sperm-oocyte coincubation with early rescue ICSI can significantly improve the clinical outcome in patients who have experienced complete failure of in vitro fertilization.</p>