Effect of iontophoresis on skin permeation of defibrase / 药学学报

ABSTRACT

<p><b>AIM</b>To investigate the effect of iontophoresis on skin permeation of defibrase.</p><p><b>METHODS</b>Iontophoresis was carried out in side-by-side chambers, excised rat skin membrane (RSM) or human epidermis membrane (HEM). The effects of electrode polarity, permeation medium pH and ionic strength were evaluated.</p><p><b>RESULTS</b>Permeation of defibrase caused by anodal iontophoresis was more effective [the apparent permeability coefficient was (1.2 +/- 0.4) x 10(-4) cm x h(-1)] than that of cathodal iontophoresis [(4.3 +/- 1.4) x 10(-5) cm x h(-1)]. The amount of permeated defibrase caused by anodal iontophoresis in pH 7.4 medium was (25 +/- 5) x 10(-14) mol x cm(-2), which was higher than that of in pH 6. 4 permeation medium [(15 +/- 4) x 10(-14) mol x cm(-2)].</p><p><b>CONCLUSION</b>Iontophoresis could enhance skin permeation of defibrase. Electroosmotic flow effect played an important role.</p>