Heparin inhibits burn-induced spleen cell apoptosis by suppressing interleukin-1 expression / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2463-2469, 2014.
Article
in English
| WPRIM
| ID: wpr-241645
ABSTRACT
<p><b>BACKGROUND</b>Epidermal burn injury may trigger significant apoptosis of the spleen cells, which might be caused by a burn-induced systemic inflammatory reaction. Heparin has been shown to possess anti-inflammatory properties. Interleukin 1 (IL-1) is centrally important among pro-inflammatory cytokines. We hypothesized that heparin might inhibit burn-induced apoptosis in the spleen via suppression of the IL-1 pathway.</p><p><b>METHODS</b>Burn injury was performed on IL-1 R+/+ ( IL-1 receptor wild-type mouse) and IL-1 R-/- (IL-1 receptor knock-out mouse) mice, and they were then treated with heparin, saline or IL-1 receptor antagonist IL-Ra. Apoptosis, IL-1α and IL-1β expression were assessed in the spleens and serum. Survival curve analysis was further applied to elucidate the mechanism of heparin's protective properties.</p><p><b>RESULTS</b>Burn induced significant apoptosis (sham 3.6%± 2.1% vs. burn 28.8%± 5.9%; P < 0.001) and remarkable expression o IL-1α and IL-1β in the mouse spleens and serum. Heparin reduced the burn-induced apoptosis in the spleens (heparin treated 8.6%± 3.4%, P < 0.005), which could be blocked by IL-1Ra. Heparin markedly decreased both IL-1α and IL-1β expression in the spleens and serum of burned mice. IL-1 R-/- mice demonstrated considerably less apoptosis in the spleens and had a higher survival rate after burns. Heparin did not significantly decrease apoptosis in the spleen and the mortality rate in IL-1 R-/- mice after burns.</p><p><b>CONCLUSION</b>Heparin inhibits burn-induced apoptosis of the spleen cells by suppressing IL-1 expression in mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Spleen
/
Burns
/
Heparin
/
Interleukin-1
/
Receptors, Interleukin-1
/
Apoptosis
/
Mice, Knockout
/
Therapeutic Uses
/
Drug Therapy
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2014
Type:
Article
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