Duodenal-jejunal bypass surgery on type 2 diabetic rats reduces the expression of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 in the thoracic aorta / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2423-2428, 2014.
Article
in English
| WPRIM
| ID: wpr-241652
ABSTRACT
<p><b>BACKGROUND</b>Bariatric surgery offers a productive resolution of type 2 diabetes mellitus (T2DM). The development of T2DM vasculopathy is due to chronic inflammation, which increases matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) expression. This study sought to examine MMP-9 and TIMP-1 expression in the thoracic aorta after duodenal-jejunal bypass (DJB) surgery on a T2DM rat model induced by a high-fat diet and low dose streptozotocin (STZ).</p><p><b>METHODS</b>Twenty-one T2DM Wistar rats induced by high-fat diet and low dose STZ were randomly divided into DJB and sham duodenal-jejunal bypass (S-DJB) groups. Ten Wistar rats were fed a normal diet as a control. Recovery of gastrointestinal function post-operation and resumption of a normal diet completed the experiment. Body weight, blood glucose, blood lipid levels, and MMP-9 and TIMP-1 expression levels in aortic endothelial cells were measured throughout.</p><p><b>RESULTS</b>DJB rats showed significant weight loss 2 weeks post-operation compared with S-DJB rats. After surgery, DJB rats showed significant improvement and steady glycemic control with improved insulin sensitivity and glucose tolerance. They also exhibited improved lipid metabolism with a decrease in fasting free fatty acids (FFAs) and triglycerides (all P < 0.05). Immunohistochemistry showed decreased MMP-9 and TIMP-1 expression 12 weeks after surgery (P < 0.01).</p><p><b>CONCLUSIONS</b>DJB surgery on an induced T2DM rat model improves blood glucose levels and lipids, following a high-fat diet and low dose STZ treatment. In addition, DJB decreased MMP-9 and TIMP-1 expression in vascular endothelial cells, which may play an important role in delaying the development of T2DM vascular disease.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta, Thoracic
/
Physiology
/
Body Weight
/
Matrix Metalloproteinase 9
/
Matrix Metalloproteinase 1
/
Diabetes Mellitus, Type 2
/
Bariatric Surgery
/
Metabolism
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS