Indian Hedgehog signaling is involved in the stretch induced proliferation of osteoblast / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 234-238, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-241819
ABSTRACT
<p><b>OBJECTIVE</b>To observe the role of the Hedgehog (Hh) genes in the proliferation of osteoblasts upon mechanical tensile strains.</p><p><b>METHODS</b>Primary osteoblasts harvested from newborn rat calvarial bone were subjected to 3% and 6% elongation of tensile stretches using Flexcell 4000 strain unit. The cultures were also treated with either recombinant N-terminals Sonic Hedgehog (N-Shh) or cyclopamine (cy), a Hh inhibitor or gadolinium (GdCl3), an inhibitor of stretch-activated channels. The proliferation of osteoblasts was quantified by cell counting, methyl thiazolyl tetrazolium (MTT) and cell cycle detection via flow cytometry. Statistical analysis was performed using SAS 8.0 software package.</p><p><b>RESULTS</b>The tensile strain, especially under 6% elongation, promoted osteoblast proliferation. Stretching force could also promote the proliferation even when the cells were treated with cy, but this effect was suppressed by GdCl3.</p><p><b>CONCLUSION</b>The induced proliferation of osteoblasts by mechanical stretched is mediated at least in part by Indian Hedgehog (Ihh) signaling.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Veratrum Alkaloids
/
Signal Transduction
/
Cell Proliferation
/
Hedgehog Proteins
Limits:
Animals
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS