Transforming growth factor beta receptor II mutations in RER positive colorectal cancers / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 6-10, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-242136
ABSTRACT
<p><b>OBJECTIVE</b>To detect alterations of microsatellite loci [transforming growth factor beta receptor II (TGF-betaRII)(A)(10), TGF-betaRII(GT)(3), hMSH3(A)(8), hMSH6(C)(8), Bax(G)(8), IGFIIR(G)(8), IGFIIR(CT)(3)] and point mutations of TGF-betaRII (TGF-betaRII 452/454, TGF-betaRII 533).</p><p><b>METHODS</b>PCR-SSLP, microdissection-PCR-SSLP, PCR-SSCP, clone sequencing and immunohistochemistry were used.</p><p><b>RESULTS</b>The mutation rate of TGF-betaRII(A)(10) in RER+ (replication error positive) colorectal carcinomas was 33% (3/9). Similar mutations were also observed in adenomas with severe dysplasia. No mutations at other microsatellite loci were found. RER+ colorectal cancers mainly occurred in male patients at a young age and were more common in the colon than in the rectum (P < 0.05).</p><p><b>CONCLUSIONS</b>RER+ colorectal cancers were found in young males and commonly located in the colon. A one third mutation rate in TGF-betaRII(A)(10) in these patients is lower than that observed in western populations, which may imply diverse pathways of carcinogenesis of RER+ colorectal carcinoma. TGF-betaRII(A)(10) mutation may play a role in the transforming process from an adenoma with severe dysplasia to a full blown carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Colorectal Neoplasms
/
Polymerase Chain Reaction
/
Protein Serine-Threonine Kinases
/
Point Mutation
/
Receptors, Transforming Growth Factor beta
/
Microsatellite Repeats
/
Genetics
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2004
Type:
Article
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