Changes of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expressions on cultured rat mesangial cells transfected with Smad 7 vector / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 544-547, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-242142
ABSTRACT
<p><b>OBJECTIVE</b>To investigate matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) expressions in the cultured rat mesangial cells (MsC) transfected with Smad 7 vector and to elucidate the mechanism of Smad 7 in blocking tissue fibrosis.</p><p><b>METHODS</b>Lipofectin method was used to transfect Smad 7 vector into MsC. Western blot and RT-PCR analyses were then used to detect Smad 7 protein and mRNA expression levels. The expressions of MMP-2 and TIMP-2 were determined by Western blot, RT-PCR and zymography assay.</p><p><b>RESULTS</b>Two MsC clones (S-22, S-26) with Smad 7 overexpression were successfully established. The two clones showed an increased expression of MMP-2 protein and enhanced enzyme activity. The expressions of TIMP-2 protein and mRNA however were suppressed.</p><p><b>CONCLUSIONS</b>It is possible that Smad 7 can alleviate the development of tissue fibrosis by upregulating the expression of MMP-2 and downregulating the expression of TIMP-2 in mesangial cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Gene Expression
/
Trans-Activators
/
Cells, Cultured
/
Blotting, Western
/
Tissue Inhibitor of Metalloproteinase-2
/
Reverse Transcriptase Polymerase Chain Reaction
/
Matrix Metalloproteinase 2
/
Cell Biology
/
DNA-Binding Proteins
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2003
Type:
Article
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