Study on the diagnostic significance of detecting the expression of AChR-gamma mRNA in rhabdomyosarcoma tissues / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 413-416, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-242162
ABSTRACT
<p><b>OBJECTIVE</b>To detect over-expression of AChR-gamma mRNA in rhabdomyosarcoma tissues by duplex RT-PCR and discuss its potential in diagnosis of rhabdomyosarcoma.</p><p><b>METHODS</b>Duplex RT-PCR was applied to the simultaneous detection of AChR-alpha and gamma subunit messenger RNA in 17 cases of rhabdomyosarcoma (9 ERMS, 6 ARMS, 2 PRMS). 20 cases of non-rhabdomyosarcomous small round cell tumors (6 poorly differentiated synovial sarcomas, 6 ES/PNET, 6 lymphomas, 2 neuroblastomas) and three normal muscle samples were also detected for AChR-alpha and gamma mRNA by the same method.</p><p><b>RESULTS</b>AChR-alpha and AChR-gamma mRNA were expressed in all the cases of rhabdomyosarcoma. The rate of quantity in both transcripts was AChR-gamma/AChR-alpha >or= 1, but the rate for three normal muscle samples was < 1. Cases of non-rhabdomyosarcomous small round cell tumors were all negative for AChR-gamma.</p><p><b>CONCLUSION</b>AChR-gamma mRNA expression detected by molecular genetic methods is useful in diagnosis and differential diagnosis of rhabdomyosarcoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rhabdomyosarcoma
/
RNA, Messenger
/
Receptors, Cholinergic
/
Receptors, Nicotinic
/
Reverse Transcriptase Polymerase Chain Reaction
/
Protein Subunits
/
Diagnosis
/
Diagnosis, Differential
/
Genetics
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2003
Type:
Article
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