Hyaluronic acid production by Streptococcus iniae and its application in rabbit skin's regeneration / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1104-1114, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-242270
ABSTRACT
Hyaluronic acid (HA) is an important biomaterial as the extracellular matrix in human body. We produced HA by fermentation of Streptococcus iniae (Strep.). Production of HA by Strep. was evaluated and further improved by strain mutation by ultraviolet. One strain with higher HA yield and lower content of protein was obtained. Its HA yield increased from (82.3±3.3) mg/L to (120±10.6) mg/L, and protein decreased from (0.178±0.011) mg/L to (0.032±0.017) mg/L. The molecular weight (MW) of HA yield from Strep. is about 3.0×10⁵ Da. Using the method of freezing and thawing, HA aqueous solution was transferred into hydrogel. This HA hydrogel, casted on sterilized non-woven fabric, was applied to repair rabbit skin with full-thickness defect. The preliminary results of the animal tests displayed that HA hydrogel obviously reduced the inflammation around the wound and promoted the skin regeneration comparing with the control tests.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Regeneration
/
Skin
/
Wound Healing
/
Biocompatible Materials
/
Hydrogels
/
Fermentation
/
Streptococcus iniae
/
Hyaluronic Acid
/
Metabolism
/
Molecular Weight
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article
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