High-level production of glucose oxidase by recombinant Pichia pastoris using a combined strategy / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 986-990, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-242281
ABSTRACT
To enhance the production of glucose oxidase by recombinant Pichia pastoris, two strategies were developed, which were namely co-feeding of methanol and sorbitol and co-expressing of the protein disulfide isomerase (PDI) and Vitreoscialla hemoglobin (VHb). The volumetric activity reached 456 U/mL by using the strain X33/pPIC9k-GOD, in 5 liter fermentator, with the co-feeding of methanol and sorbitol, it was 0.2 fold higher than that only feeding by methanol. The improved strain was obtained by co-expressing PDI-VHb with GOD. While fermented in a 5 liter fermentator by feeding methanol and sorbitol, the activity of the improved strain reached 716 U/mL with a yield of 7 400 mg/L total soluble protein concentration. These results indicated that heterologous protein expression level can be enhanced by optimizing fermentation condition and co-expression molecular chaperon in Pichia pastoris.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Sorbitol
/
Recombinant Proteins
/
Bioreactors
/
Methanol
/
Fermentation
/
Glucose Oxidase
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article
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