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Effect of integration loci of genome on heterologous gene expression in Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology ; (12): 901-911, 2016.
Article in Chinese | WPRIM | ID: wpr-242288
ABSTRACT
Chromosomal integration of heterologous genes or pathways is preferred over the use of episomal plasmids for its inherently stability and thus more desirable in the industrial setting. However, the position of integration of heterologous genes in the genome influences the expression levels. In combination of high throughput transformation of the Yeast Knock-out Collection (YKO) and FACS analysis, the position effect on heterologous reporter gene gfp was identified across the whole genome in yeast. In total 428 high-expressed sites and 444 low-expressed sites were spotted, providing massive data to analyze patterns and reasons for region dependency of gene expression on the genome-wide scale.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Saccharomyces cerevisiae / Gene Expression Regulation, Fungal / Genome, Fungal / Genes, Reporter / Gene Knock-In Techniques / Genetics Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Saccharomyces cerevisiae / Gene Expression Regulation, Fungal / Genome, Fungal / Genes, Reporter / Gene Knock-In Techniques / Genetics Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2016 Type: Article