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Effect of ribosome engineering on butenyl-spinosyns synthesis of Saccharopolyspora pogona / 生物工程学报
Chinese Journal of Biotechnology ; (12): 259-263, 2016.
Article in Zh | WPRIM | ID: wpr-242295
Responsible library: WPRO
ABSTRACT
Through introducing mutations into ribosomes by obtaining spontaneous drug resistance of microorganisms, ribosome engineering technology is an effective approach to develop mutant strains that overproduce secondary metabolites. In this study, ribosome engineering was used to improve the yield of butenyl-spinosyns produced by Saccharopolyspora pogona by screening streptomycin resistant mutants. The yields of butenyl-spinosyns were then analyzed and compared with the parent strain. Among the mutants, S13 displayed the greatest increase in the yield of butenyl-spinosyns, which was 1.79 fold higher than that in the parent strain. Further analysis of the metabolite profile of S13 by mass spectrometry lead to the discovery of Spinosyn α1, which was absent from the parent strain. DNA sequencing showed that there existed two point mutations in the conserved regions of rpsL gene which encodes ribosomal protein S12 in S13. The mutations occurred a C to A and a C to T transversion mutations occurred at nucleotide pair 314 and 320 respectively, which resulted in the mutations of Proline (105) to Gultamine and Alanine (107) to Valine. It also demonstrated that S13 exhibited genetic stability even after five passages.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Ribosomal Proteins / Ribosomes / Genetic Engineering / Saccharopolyspora / Point Mutation / Macrolides / Genetics / Metabolism Language: Zh Journal: Chinese Journal of Biotechnology Year: 2016 Type: Article
Full text: 1 Index: WPRIM Main subject: Ribosomal Proteins / Ribosomes / Genetic Engineering / Saccharopolyspora / Point Mutation / Macrolides / Genetics / Metabolism Language: Zh Journal: Chinese Journal of Biotechnology Year: 2016 Type: Article