Production of sugar syrup containing rare sugar using dual-enzyme coupled reaction system / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 90-97, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-242409
ABSTRACT
Enzymatic conversion is very important to produce functional rare sugars, but the conversion rate of single enzymes is generally low. To increase the conversion rate, a dual-enzyme coupled reaction system was developed. Dual-enzyme coupled reaction system was constructed using D-psicose-3-epimerase (DPE) and L-rhamnose isomerase (L-RhI), and used to convert D-fructose to D-psicose and D-allose. The ratio of DPE and L-RhI was 110 (W/W), and the concentration of DPE was 0.05 mg/mL. The optimum temperature was 60 degrees C and pH was 9.0. When the concentration of D-fructose was 2%, the reaction reached its equilibrium after 10 h, and the yield of D-psicose and D-allose was 5.12 and 2.04 g/L, respectively. Using the dual-enzymes coupled system developed in the current study, we could obtain sugar syrup containing functional rare sugar from fructose-rich raw material, such as high fructose corn syrup.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Temperature
/
Carbohydrate Epimerases
/
Chemistry
/
Aldose-Ketose Isomerases
/
Fructose
/
Glucose
/
Hydrogen-Ion Concentration
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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