Improved extraction of solanesol from tobacco waste by enzymatic cell wall breaking / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1706-1710, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-242419
ABSTRACT
To improve the extraction of solanesol from tobacco waste, we developed an enzymatic cell wall-breaking process with combined cellulase and ligninase. The effects of reaction time, temperature, pH and enzyme/substrate ratio were determined. The results show that the catalytic effect was better than either single enzyme when the ratio of cellulase to ligninase was 151 (U/U). Under the optimized conditions of 175 U/g (enzymes/substrate), tobacco to water 15 (W/W), temperature 40 degrees C and pH 6.0, the concentration of solanesol in the solution could reach 0.33 g/L after 8 h. And the average leaching rate reached 96.53% which was 1.68 times of the extraction methods of chemical reflux. It provides new way for the extraction of solanesol from tobacco waste, and worthwhile to be further explored.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygenases
/
Terpenes
/
Tobacco
/
Cell Wall
/
Cellulase
/
Chemistry
/
Plant Leaves
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
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