A method to determine the structure of the complex of enzyme and its substrate using 6-phosphate-beta-glucosidase at low temperature / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1828-1835, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-242449
ABSTRACT
To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C, we soaked the crystal of 6-phosphate-beta-glucosidase (Bg1A) in the liquor containing p-nitrophenyl-beta-D-glucopyranoside-6-phosphate (pNPbetaG6P). The diffraction data at 2 A resolution was collected and an intact and unambiguous electron density map of pNPbetaG6P was obtained. These results provide an effective method for the research of cryoenzymology and the intermediate state of enzyme-substrate complex in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Substrate Specificity
/
X-Ray Diffraction
/
Chemistry
/
Cold Temperature
/
Crystallization
/
Glucosidases
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
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