Stable and efficient expression of hepatitis B virus S antigen and preS1 epitope fusion protein (S/preS1) in CHO cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1808-1816, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-242451
ABSTRACT
Hepatitis B surface antigen (HBsAg) carrying preS sequences could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. Here we report the success in achieving efficient and stable expression of hepatitis B virus S antigen and preS1 epitope fusion protein (S/preS1) in CHO cells. The HMRCHEF53u/Neo-S/preS1 expression vector carrying S/preS1 gene was constructed and transfected into CHO-S cells. A stable and high-expression CHO cell line, named 10G6, was selected by ELISA and limiting dilution analysis. Western blotting analysis showed S/preS1 expressed from 10G6 cells possessed both S and preS1 antigenicity. 10G6 cells displayed characters of favorable growth and stable S/preS1 expression in repeated batch cultures as evaluated by viable cell density, viability and S/preS1 concentration. And cultivation of 10G6 cells in fed-batch mode resulted in S/preS1 production at 17-20 mg/L with viable cell density at 7 x 10(6)-10 x 10(6) cells/mL.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Precursors
/
Recombinant Fusion Proteins
/
Transfection
/
Hepatitis B virus
/
Cricetulus
/
CHO Cells
/
Hepatitis B Vaccines
/
Allergy and Immunology
/
Genetics
/
Hepatitis B Surface Antigens
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
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