Construction and fermentation control of reductive TCA pathway for malic acid production in Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1484-1493, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-242463
ABSTRACT
Malic acid is widely used in food, and chemical industries. Through overexpressing pyruvate carboxylase and malate dehydrogenase in pdc1-deficient Saccharomyces cerevisiae, malic acid was successfully produced through the reductive TCA pathway. No malic acid was detected in wild type Saccharomyces cerevisiae, however, 45 mmol/L malic acid was produced in engineered strain, and the concentration of byproduct ethanol also reduced by 18%. The production of malic acid enhanced 6% by increasing the concentration of Ca2+. In addition, the final concentration reached 52.5 mmol/L malic acid by addition of biotin. The increasing is almost 16% higher than that of the original strain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidation-Reduction
/
Pyruvate Carboxylase
/
Saccharomyces cerevisiae
/
Industrial Microbiology
/
Signal Transduction
/
Citric Acid Cycle
/
Metabolic Networks and Pathways
/
Fermentation
/
Metabolic Engineering
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
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