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Construction of an ethanologenic Escherichia coli strain expressing beta-glucosidase / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1254-1267, 2013.
Article in Chinese | WPRIM | ID: wpr-242484
Responsible library: WPRO
ABSTRACT
Constructing ethanologenic strains with cellulose activity is important to achieve consolidated bioprocessing of lignocellulose for ethanol production. In this study, we integrated the pyruvate decarboxylase gene pdc and alcohol dehydrogenase gene adhB from Zymomonas mobilis ZM4 into Escherichia coli JM109 by Red recombination method to generatea recombinant strain E. coli P81 that could produce ethanol from glucose. Abeta-glucosidase gene bglB from Bacillus polymyxa 1.794 was cloned into the recombinant E. coli P81 and beta-glucosidase was expressed to give a new recombinant strain E. coli P81 (pUC19-bglB) with dual functions of cellobiose degradation and ethanol production. The extracellular beta-glucosidaseactivity was 84.78 mU/mL broth and the extracellular cellobiase activity of E. coli P81 (pUC19-bglB) was 32.32 mU/mL broth. E. coli P81 (pUC19-bglB) fermented cellobiose to ethanol with a yield of 55.8% of the theoretical value, and when glucose and cellobiose were co-fermented, the ethanol yield reached 46.5% of thetheoretical value. The construction of consolidated bioprocessing strain opens the possibility to convert cellobiose to ethanol in a single bioprocess.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Recombinant Proteins / Cellulose / Beta-Glucosidase / Ethanol / Escherichia coli / Fermentation / Bacterial Secretion Systems / Genetics / Metabolism Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Recombinant Proteins / Cellulose / Beta-Glucosidase / Ethanol / Escherichia coli / Fermentation / Bacterial Secretion Systems / Genetics / Metabolism Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2013 Type: Article