Effects of gene design on recombinant protein expression: a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1201-1213, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-242489
ABSTRACT
It has become a hotspot and keystone in gene engineering and bioengineering to produce recombinant proteins through heterologous expression systems. Unfortunately, not all the genes could be successfully and effectively expressed in heterologous hosts. The role of gene itself in regulating translation process through its intrinsic sequence characteristics such as codon bias, codon pair bias, GC content, mRNA secondary structure and mRNA stability, has been gradually elucidated. Here we review these factors that influence the translation processes and their corresponding optimization methods in the process of gene design. We emphatically discussed codon bias and codon pair bias and their optimization methods. In particular, the latest theories of codon harmonization and codon pair harmonization were discussed and compared with the traditional codon and codon pair optimization strategies in gene design.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Biosynthesis
/
Recombinant Proteins
/
Codon
/
DNA, Bacterial
/
Protein Engineering
/
RNA Stability
/
Escherichia coli
/
Genes, Synthetic
/
Genetics
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS