Cloning of human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor from phage Fab antibody library / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To screen human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor.</p><p><b>METHODS</b>A synthesized polypeptide containing 23 amino acid residues of the gp120 antigen epitope binding chemokine receptor was coated as the solid-phase antigen. After biopanning from the HIV-1 phage Fab antibody library, the acquired positive clones were tested and sequenced.</p><p><b>RESULTS</b>One clone of human phage Fab monoclonal antibody against HIV-1 gp120 polypeptide was acquired. It has high affinity, specificity and inhibition rate and it belongs to IgG I subclass and kappa type. Its Vh H and V kappa were derived from Vh III and V kappa III.</p><p><b>CONCLUSIONS</b>The human phage Fab fragment against HIV-1 gp120 antigen site binding chemokine receptor was acquired.</p>