Expression of full-length hepatitis E virus structural gene using baculovirus expression system / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To obtain recombinant baculovirus containing full-length structural gene of HEV and to express HEV structural protein.</p><p><b>METHODS</b>Full-length structural gene of HEV (5147-7126 nt) was inserted into baculovirus expression vector pAcUW51. The recombinant plasmid and Baculo Gold DNA were co-transfected into insect cell line Sf9. Single plaque was picked and amplified and expression of HEV structural protein was tested by SDS-PAGE, Western blot and immunofluorescence methods.</p><p><b>RESULTS</b>SDS-PAGE analysis showed HEV structural protein was highly expressed; Western blot and immunofluorescence assay showed that the expression product could specifically react with HEV positive sera, confirming the protein possessing HEV specific antigenicity.</p><p><b>CONCLUSIONS</b>HEV structural protein was successfully expressed using baculovirus expression system.</p>