Construction of recombinant vaccinia virus co-expressing mutant E6 plus E7 proteins and detection of its immunogenicity and antitumor response / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 341-344, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-242615
ABSTRACT
<p><b>OBJECTIVE</b>To generate a candidate HPV16 vaccine for experimental and therapeutical use for cervical cancer.</p><p><b>METHODS</b>The mutants of HPV16 early E6 and E7 genes were inserted into a vaccinia virus expression vector. A strain of recombinant vaccinia virus was constructed through homologous recombination.</p><p><b>RESULTS</b>Showed that the mutant E6 and E7 genes were located at TK gene region of vaccinia virus Tiantan strain in a head to head orientation under the control of early/late promoters, H6 and 7.5K respectively. Studies in mice indicated that VmE6E7 could elicit specific antibodies against E6 and E7, and retarded or prevented tumor development in a proportion of C57 BL/6 mice challenged by syngeneic HPV16E6 and E7 transformed tumor cells.</p><p><b>CONCLUSIONS</b>The success in constructing VmE6E7 provides a basis for the further development of HPV16 therapeutic vaccine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Recombination, Genetic
/
Repressor Proteins
/
Vaccinia virus
/
Transfection
/
Oncogene Proteins, Viral
/
Allergy and Immunology
/
Papillomavirus E7 Proteins
/
Genes, Viral
/
Genetic Vectors
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2002
Type:
Article
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