Preparation of gene chip for detecting different expression genes involved in aflatoxin biosynthesis / 中华预防医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop the methodology of gene chip to analyse genes involved in aflatoxin biosynthesis.</p><p><b>METHODS</b>In comparing reversed transcriptional PCR with gene chip, the gene chip was used to detect genes involved in aflatoxin biosynthesis.</p><p><b>RESULTS</b>After arrayed the slide was incubated in water for 2 hours, exposed to a 650 mJ/cm2 of ultraviolet irradiation in the strata-linker for 30 s, roasted under 80 degrees C for 2 hours in oven, pre-hybridized for 45 minutes and dealt with other procedures. Finally, the slide was hybridized with fluor-derivatized sample at 42 degrees C for 16 hours.</p><p><b>CONCLUSION</b>With the reasonable probe design and applicable protocol, the gene chip was prepared effectively for research on genes involved in aflatoxin biosynthesis.</p>