Heterozygous genotypes and molecular characteristics of Organophosphorus resistance associated esterase B2 genes of Culex pipiens complex / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 390-394, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-242644
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the heterozygous genotype and molecular characteristics of Organophosphorus resistance associated with heterozygous Estbeta2 of esterase B2 gene from natural population of Culex pipiens complex.</p><p><b>METHODS</b>Genomic DNA was extracted from natural populations of Culex pipiens complex in Hangzhou. The PCR-restriction fragment length polymorphism (PCR-RFLP) assay was applied to type the resistance associated esterase gene. Estbeta2 of esterase B2 gene was identified by PCR-RFLP, and the genotyping for heterozygous Estbeta2 was carried out after restriction enzyme digesting by Bfm I endonuclease.</p><p><b>RESULTS</b>The DNA was isolated from 207 Culex pipiens respectively, while 156 PCR samples showed positive and the positive rate was 75.36% (156/207). The PCR-RFLP assay of esterase B2 gene revealed that the Estbeta2 was accounted about 28.20% (44/156) in 156 positive samples. There were two genotypes identified, namely homozygous Estbeta2 (90.90%, 30/33) and heterozygous Estbeta2 (9%, 3/33), heterozygous Estbeta2 was in existence of a hybrid form as which combined with Estbeta2 and a subtype (Estbeta2/Estbeta2(1)).</p><p><b>CONCLUSION</b>Heterozygous Estbeta2 of Organophosphorus resistance associated with esterase genotype was determined in natural population of Culex pipiens, and a genotyping method was established.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organophosphorus Compounds
/
Pharmacology
/
Phenotype
/
Insecticide Resistance
/
Serine Endopeptidases
/
Genes, Insect
/
Culex
/
Genetics
/
Genotype
/
Heterozygote
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS