Anti-atherosclerotic effect of betaine in apolipoprotein E-deficient mice / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 742-747, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-242699
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of betaine on the formation of atherosclerotic plaque in apolipoprotein E (ApoE)-deficient mice and explore its anti-inflammatory mechanism.</p><p><b>METHODS</b>Seven-week-old ApoE-deficient mice (C57BL/6J background) were divided into four groups randomly based on body weight model group and three betaine groups. Wild-type mice with the same age and genetic background were used as control group. The control group and model group were fed AIN-93G diet. Three betaine groups were fed AIN-93G diet supplemented with 1, 2, 4 g betaine/100 g diet, respectively. Serum tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein-1, lipid levels and methylation status of TNF-alpha promotor in aorta were determined at 0, 7 and 14 weeks. The percentage of aorta sinus plaque to lumen area was measured at 14-week.</p><p><b>RESULTS</b>The percentage of aorta sinus plaque to lumen area of 1% and 2% betaine groups were (11.43+/-2.65)% and (12.09+/-3.07)%, respectively, which were 41% and 33% smaller than that of the model group (t=3.117, 3.010, respectively, and P<0.01). Serum TNF-alpha level of three betaine groups were (56.33+/-3.86), (63.04+/-4.67) and (65.52+/-3.97) pg/ml, respectively, which were lower than that of the model group (79.40+/-4.68) pg/ml (t=9.270, 6.571 and 5.576, respectively, P<0.001), but there was no significant difference in the methylation status of TNF-alpha promotor among all five groups.</p><p><b>CONCLUSION</b>Betaine could inhibit the development of atherosclerosis via anti-inflammation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apolipoproteins E
/
Pharmacology
/
Betaine
/
Blood
/
Tumor Necrosis Factor-alpha
/
Chemokine CCL2
/
Dietary Supplements
/
Therapeutic Uses
/
Disease Models, Animal
/
Drug Therapy
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2008
Type:
Article
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