False human immunodeficiency virus test results associated with rheumatoid factors in rheumatoid arthritis / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 103-106, 2014.
Article
in English
| WPRIM
| ID: wpr-242890
ABSTRACT
<p><b>OBJECTIVE</b>To investigate if immunological factors associated with rheumatoid arthritis (RA) affect the result of human immunodeficiency virus (HIV) screening by electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA).</p><p><b>METHODS</b>100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors (RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA.</p><p><b>RESULTS</b>The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method (P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA (P<0.01).</p><p><b>CONCLUSIONS</b>Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Arthritis, Rheumatoid
/
Rheumatoid Factor
/
Blood
/
Enzyme-Linked Immunosorbent Assay
/
HIV Antibodies
/
HIV Antigens
/
HIV Infections
/
Diagnosis
/
False Positive Reactions
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Humans
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2014
Type:
Article
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