Culture and identify the human umbilical vein endothelial cells and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains in the cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 653-656, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-242927
ABSTRACT
<p><b>OBJECTIVE</b>To study the cultural method and identification of human umbilical vein endothelial cells (HUVECs), and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains(Tie-2) in HUVECs.</p><p><b>METHODS</b>HUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by VIII monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and SABC immunocytochemistry.</p><p><b>RESULTS</b>HUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85%.</p><p><b>CONCLUSION</b>Highly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Umbilical Veins
/
Immunoglobulins
/
Cells, Cultured
/
Endothelial Cells
/
TYK2 Kinase
/
Human Umbilical Vein Endothelial Cells
/
EGF Family of Proteins
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2009
Type:
Article
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