Establishment and application of an in vitro model for apatite crystal mineralization / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish an in vitro model for the apatite crystal mineralization. To evaluate the influences of bovine serum albumin (BSA) and fluoride to the mineralization of apatite crystal.</p><p><b>METHODS</b>The model was constructed using cation selective membrane (CMV) and dialysis membrane. Double distilled water (DDW), BSA, 5, 20, 100 mg x L(-1) fluoride were added into the reaction space of the model. Reaction was carried out at 37 degrees C for 3 days under gentle stirring. The crystals were identified by scanning electron microscope (SEM) and X-ray diffraction (XRD).</p><p><b>RESULTS</b>The model was established successfully. When DDW and BSA were added respectively, the main component of the deposit was octacalcium phosphate (OCP), but the shape and size of the crystals differs from each other. When fluoride with different concentration were added, the main component of the crystal turned to rod-like and prism-like fluoroapatite (FAP) crystal. The size and crystallinity of the FAP increased with the increase of the fluoride concentration.</p><p><b>CONCLUSION</b>It is an effective way to evaluate the influence factors of the apatite crystal mineralization by using the in vitro model.</p>