Heterotopic complex membrane induces bone formation / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 357-360, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-243004
ABSTRACT
<p><b>OBJECTIVE</b>The composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen (Co), polylactic-co-glycolic acid (PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane.</p><p><b>METHODS</b>48 male Kunming adult mice were divided into two groups randomly Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination.</p><p><b>RESULTS</b>Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X-ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane.</p><p><b>CONCLUSION</b>Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Polyesters
/
Polyglycolic Acid
/
Polymers
/
Recombinant Proteins
/
Transforming Growth Factor beta
/
Collagen
/
Lactic Acid
/
Bone Morphogenetic Protein 2
/
Glycolates
Limits:
Animals
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2009
Type:
Article
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