Generation of transgene-free induced pluripotent stem cells with non-viral methods / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 50-54, 2013.
Article
in English
| WPRIM
| ID: wpr-243219
ABSTRACT
Induced pluripotent stem (iPS) cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors (Oct3/4, Sox2, Klf4, and c-Myc). The technique was quickly reproduced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic potential in animal models of sickle cell anemia and Parkinson's disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Transduction, Genetic
/
Transfection
/
Transgenes
/
Cellular Reprogramming
/
Induced Pluripotent Stem Cells
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2013
Type:
Article
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