PTEN and PDCD4 are bona fide targets of microRNA-21 in human cholangiocarcinoma / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 65-72, 2012.
Article
in English
| WPRIM
| ID: wpr-243263
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells.</p><p><b>METHODS</b>The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis.</p><p><b>RESULTS</b>Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile duct tissues (P<0.05). The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholangiocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN (P<0.01) and PDCD4 (P<0.05) and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue.</p><p><b>CONCLUSION</b>microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Bile Duct Neoplasms
/
Bile Ducts, Intrahepatic
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
RNA-Binding Proteins
/
Cholangiocarcinoma
/
Gene Expression Profiling
/
MicroRNAs
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2012
Type:
Article
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