Laboratory study on a rare case of chronic myeloid leukemia with ins(22;9)t(9;13) and Ph-negative / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 355-358, 2010.
Article
in Zh
| WPRIM
| ID: wpr-243356
Responsible library:
WPRO
ABSTRACT
The study aimed to examine a rare case of Philadelphia (Ph)-negative chronic myeloid leukemia (CML) with t(9;13). Chromosome samples were prepared after culture of bone marrow cells for 24 hours, the karyotypes were analyzed by G banding technique. Chromosome painting analysis was performed by using whole chromosome paints for chromosomes 9 and 22. Fluorescence in situ hybridization (FISH) was done with dual color dual fusion LSI bcr/abl probe. Bcr/abl transcripts were detected by real time fluorescence quantitative polymerase chain reaction (RQ-PCR). As a result, G banding analysis showed a karyotype of 45, XX, der(9)t(9;13)(q34;q10), -13[20]. FISH assay using LSI bcr/abl DNA probe showed a red abl signal inserted into der(22) and a fusion signal of bcr/abl rearrangement was discovered. RQ-PCR detected high copies of bcr/abl transcripts. In conclusion, insertion of bcr/abl rearrangement was a rare variant t(9;22) and could be well detected by molecular techniques, however, regular cytogenetic banding technique and whole chromosome paintings may probably lead a misdiagnosis to such cases.
Full text:
1
Index:
WPRIM
Main subject:
Chromosomes, Human, Pair 9
/
Chromosomes, Human, Pair 13
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Chromosomes, Human, Pair 22
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Chromosome Painting
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Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative
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Genetics
/
Karyotyping
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article