High-dose heat shock protein gp96 immunization prevents type 1 diabetes via inducing regulatory T cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1685-1693, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-243689
ABSTRACT
Type 1 diabetes (T1D), the most prevalent human autoimmune disease, occurs in genetically susceptible individuals. Regulatory T cells (Tregs) are defective in T1D setting. Therefore, efforts to repair or restore Tregs in T1D may prevent or reverse this autoimmune disease. Here, we studied the potential role of rgp96 in preventing T1D, using non-obese diabetic (NOD) mice as an animal model. High-dose rgp96 immunization elicited efficient protection of mice against T1D, as evidenced by stable blood glucose, decreased disease incidence. Significantly increased CD4⁺ CD25⁺ Foxp3⁺ Tregs were observed in immunized mice. In vitro co-culture experiments demonstrated that rgp96 stimulation enhanced Treg proliferation and suppressive function by up-regulation of Foxp3 and IL-10. Our work shows that activation of Tregs by high-dose rgp96 immunization protects against T1D via inducing regulatory T cells and provides preventive and therapeutic potential for the development of an rgp96-based vaccine against T1D.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Up-Regulation
/
Vaccination
/
Mice, Inbred NOD
/
Interleukin-10
/
T-Lymphocytes, Regulatory
/
Coculture Techniques
/
Diabetes Mellitus, Type 1
/
Allergy and Immunology
/
Forkhead Transcription Factors
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS