Experimental study of the enhancement effect of aminopeptidase N inhibitor ubenimex on the differentiation induction activity of all-trans-retinoic acid in acute promyelocytic leukemia cells and its mechanism / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 445-448, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-243933
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of aminopeptidase N inhibitor ubenimex on differentiation induction of all-trans-retinoic acid (ATRA) in acute promyelocytic leukemia (APL) cells and its mechanism.</p><p><b>METHODS</b>The expression of CD11b was analyzed by flow cytometry and nitroblue-tetrazolium (NBT) reduction assay was performed to determine the cell differentiation of APL cells. The expressions of c-Myc, ERK1/2, p38MAPK protein and the phosphorylation of ERK1/2, p38MAPK protein in NB4 cells were detected by Western blot assay.</p><p><b>RESULTS</b>Ubenimex alone induced no significant changes in NBT reduction activity and CD11b expression but potentiated the differentiation induction activity of ATRA in APL cells. 100 microg/ml of ubenimex could enhance the NBT reduction activity induced by 10 nmol/L of ATRA, intensify the down-regulation of c-Myc protein expression and inhibit the phosphorylation of p38MAPK protein induced by 10 nmol/L of ATRA in NB4 cells.</p><p><b>CONCLUSIONS</b>Ubenimex could potentiate ATRA induced differentiation in APL cells, which may be correlated with the inhibition of p38 MAPK protein phosphorylation and regulation of c-Myc protein expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Tretinoin
/
In Vitro Techniques
/
Leukemia, Promyelocytic, Acute
/
Cell Differentiation
/
Drug Synergism
/
Flow Cytometry
/
Aminopeptidases
/
Leucine
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article
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