The proteomics study of apoptotic NB4 cells induced by sodium butyrate / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 436-440, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-243935
ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular mechanisms of apoptotic NB4 cells induced by the histone deacetylase inhibitor, sodium butyrate(SB).</p><p><b>METHODS</b>SB was exposed to NB4 cells at a final concentration of 1.0 mmol/L. The untreated and treated cells were analysed with FACS and 2-dimensional electrophoresis (2-DE) at 0, 12, 24, 48 and 72 h. The changed protein spots were identified with MALDI-TOF-MS and ESI-TOF-MS/MS.</p><p><b>RESULTS</b>SB induced apoptosis of NB4 cells. Twenty-one changed proteins involving apoptotic signal transduction, immunological regulation, transcriptional control, cellular metabolism, molecular transport and so on were identified. Thirteen of them had been reported to be related to apoptosis.</p><p><b>CONCLUSION</b>SB can induce apoptosis and many functional protein changes in tumor cells. These results pave the way to further explore the anti-tumor mechanism of SB.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Butyrates
/
Apoptosis
/
Proteomics
/
Cell Line, Tumor
/
Flow Cytometry
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article
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