Effect of shRNA-mediated survivin gene silencing on apoptosis and proliferation of leukemia cell line / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 394-397, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-243939
ABSTRACT
<p><b>OBJECTIVE</b>To transfect a short hairpin RNA (shRNA) against survivin gene into human T lymphoblastic leukemia cell line Jurkat, and to explore the effects on apoptosis and proliferation of transfected cells.</p><p><b>METHODS</b>The survivin-shRNA expression vector were constructed and transfected into Jurkat cells. Expression of survivin mRNA and protein were assessed by RT-PCR and Western blot analysis respectively. Apoptosis index of transfected Jurkat cells was quantified by flow cytometry. The potential of cell proliferation was described by cell growth curves.</p><p><b>RESULTS</b>In survivin-shRNA transfected Jurkat cells, survivin mRNA levels were significantly reduced by 66.67% ( transient transfection) and 60.69% ( stable transfection) respectively, compared with that in control-shRNA treated group and PBS treated group (P < 0.05); and the levels of survivin protein were significantly reduced by 63.41% (transient transfection) and 60.18% (stable transfection), compared with that in the two control groups (P < 0.05). Apoptosis index was significantly increased during both transient and stable transfection, respectively [(22. 41 +/- 2.83)% and (20.73 +/- 2.56)% (P < 0.05)]. Survivin-shRNA also inhibited the proliferation of Jurkat cells.</p><p><b>CONCLUSIONS</b>Vector-based survivin-shRNA can effectively reduce the expression of survivin gene, induce apoptosis</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Gene Expression
/
Apoptosis
/
Jurkat Cells
/
Gene Silencing
/
RNA, Small Interfering
/
RNA Interference
/
Cell Proliferation
/
Inhibitor of Apoptosis Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article
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