Expression of CD66c (CEACM6) in adult acute leukemia and its significance / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 370-373, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-243945
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of CD66c (CEACM6) in adult acute leukemia and its significance.</p><p><b>METHODS</b>Acute leukemia cell lines HL-60, K562, LCL721.221 and Jurkat were cultured in vitro. RT-PCR and multi-parameter flow cytometry were applied to analysis of CD66c mRNA and protein expression respectively in the cell lines and patient' s bone marrow leukemic cells. Cytogenetic analysis for 199 bone marrow samples from leukemia patients and Minimal Residual Disease (MRD) detection for 25 CD66c positive B lineage ALL were performed.</p><p><b>RESULTS</b>(1) CD66c expression both on cell surface and in plasma were negative in all the cell lines. (2) Four of 127 AML (3.15%) (mainly of M2 and M4), and 28 of 79 ALL (35.44%) (all of B linage ALL) were CD66c positive the subtypes of the ALL being common B-ALL (20/54) and pre B-ALL (8/11) including 8 Ph + B-linage ALL. (3) Six-month relapse rate was significantly different between the MRD positive and negative patients. (4) CD66c mRNA was strongly expressed in B-linage ALL. For the cell lines, only the HL60 cells weakly expressed CD66c mRNA.</p><p><b>CONCLUSION</b>CD66c expression could be a useful bio-marker for the MRD analysis in ALL, and is closely associated with its transcription level.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Leukemia, Myeloid, Acute
/
Carcinoembryonic Antigen
/
Antigens, CD
/
Cell Adhesion Molecules
/
Neoplasm, Residual
/
HL-60 Cells
/
K562 Cells
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
GPI-Linked Proteins
Limits:
Adolescent
/
Adult
/
Aged
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article
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