Study of genes involved in chronic myeloid leukemia with t (3; 21) (q26; q22) in blastic crisis / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 310-313, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-243955
ABSTRACT
<p><b>OBJECTIVE</b>To explore genes involved in chronic myeloid leukemia (CML) with t (3; 21) (q26; q22) chromosome translocation in blastic crisis.</p><p><b>METHODS</b>A case of CML patient with t (3; 21) (q26; q22) in blastic crisis was reported. AML1 and bcr/abl genes were detected by FISH in interphase and metaphase cells. Genes involved in t (3; 21) (q26; q22) were analysed by RT-PCR and sequencing.</p><p><b>RESULTS</b>AML1 gene hybridization signal was detected in der (3) and der (21) chromosomes. AML1-Evi1, AML1-MDS1-Evi1, AML1-EAP fusion transcripts and Evi1 gene were detected in mRNA level, but no AML1-Evi1 fusion transcript. The mRNA expression level of AML1-MDS1-Evi1 fusion gene was 1.58 and 1.54 times higher than that of AML1-MDS1 and AML1-EAP, respectively. The mRNA expression level of Evi1 gene of the patient was 2.71 times higher than that of HEL cell line.</p><p><b>CONCLUSION</b>t (3; 21) (q26; q22) resulted in the AML1-MDS1-Evi1, AML1-MDS1, AML1-EAP fusion transcripts, and Evi1 gene was also activated by the translocation. These secondary aberrations should be the molecular basis of CML patient with t (3; 21) (q26; q22) in blastic crisis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Transcription Factors
/
Translocation, Genetic
/
Chromosomes, Human, Pair 3
/
Chromosomes, Human, Pair 21
/
Proto-Oncogenes
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Blast Crisis
/
Oncogene Proteins, Fusion
/
Fusion Proteins, bcr-abl
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2006
Type:
Article
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