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Study on mismatch repair genes of chronic myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology ; (12): 103-106, 2006.
Article in Chinese | WPRIM | ID: wpr-243986
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and regulation mechanism of mismatch repair (MMR) genes in chronic myeloid leukemia (CML).</p><p><b>METHODS</b>Expression of MMR genes hMSH2, hMSH3, hMSH6, hMLH1 and hPMS2 mRNAs in 62 CML patients and K562 cell line were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of bcr-abl mRNA and MMR genes mRNA were detected by RT-PCR in 26 CML patients with allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients on imatinib treatment. Expression of bcr-abl mRNA was detected by RT-PCR and tyrosine phosphorylation of BCR-ABL fusion protein by Western blot.</p><p><b>RESULTS</b>Expression of hMSH2, hMSH3 and hMLH1 mRNA was significantly lower in CML and K562 cells than in normal control (P < 0.05). In 26 CML with allo-PBSCT and 4 CML patients on imatinib treatment, expressions of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while expression of bcr-abl mRNA decreased. In CML MNC after imatinib treatment and in K562 cells, expression of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while tyrosine phosphorylation of BCR-ABL fusion protein decreased.</p><p><b>CONCLUSION</b>Expressions of hMSH2, hMSH3 and hMLH1 mRNA were down-regulated by bcr-abl fusion gene.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Piperazines / Pyrimidines / Benzamides / RNA, Messenger / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Gene Expression / Gene Expression Regulation, Neoplastic / Fusion Proteins, bcr-abl / K562 Cells Limits: Adult / Aged / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Hematology Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Piperazines / Pyrimidines / Benzamides / RNA, Messenger / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Gene Expression / Gene Expression Regulation, Neoplastic / Fusion Proteins, bcr-abl / K562 Cells Limits: Adult / Aged / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Hematology Year: 2006 Type: Article